Plasmid_Backbone

Part:BBa_J99000

Designed by: David Bikard   Group: Mazel Lab   (2009-12-17)


pSWK oriTRP4; [CmR]; oriVR6K; lambda_attP

This vector has the conditional origin of replication oriVR6K and can be maintained only in strains expressing the pir gene. It carries the origin of transfer of RP4 and can thus be used in conjugation assays in a donor cell expressing the RP4 conjugation machinery. It also carries the attP recombination site of the lambda phage in order to permit site specific integration at the attB site of E.coli chromosome mediated by the lambda integrase (Valens et al., 2004).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2215
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2209
    Illegal BglII site found at 917
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2209
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2209
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2224
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2178
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
n/apSWK oriTRP4; [CmR]; oriVR6K; lambda_attP